Test Catalog



Also known as: RENIN, E757, RENIN ACT, REN
Orderable Code: E757
Test Contains (LOINC): Renin Activity (2915-7)
Sample Type: Plasma
Preferred Container: EDTA (Lavender Top) Tube
Alternate Container: K2EDTA (Pink)
Sample Volume: 2.0 mL
Minimal Sample Volume: 1.2 mL
Handling Instructions
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Sample should be collected mid-morning after the patient has been active for at least two hours with the patient seated for 5-15 minutes. Collect specimen per tube manufacturer’s instructions. Centrifuge sample and separate plasma from cells ASAP. Aliquot and freeze.
Specimens should not be collected from patients taking spironolactone.
Client Transport Temperature: Critical Frozen
Specimen Stability: Frozen: 2 Months
Unsuitable Specimen: Refrigerated samples. Serum. Specimens collected in citrate, heparin, or oxalate. Grossly hemolyzed, icteric, or lipemic specimens.
Frequency: Monday, Tuesday, Wednesday, Thursday, Friday
Expected Turn Around: 2 – 4 Days
Responsible Dept: Reference Lab
Referral Lab: SRL
Referral Lab Code: E757
CPT: 84244
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Renin is a hormone enzyme central to the renin-angiotensin system responsible for regulating blood pressure, blood volume, and electrolyte balance. Produced by the cleavage of prorenin in the juxtaglomerular cells of the kidney when renal blood flow is reduced, renin is secreted directly into circulation where it converts angiotensinogen to angiotensin I. Angiotensin converting enzyme (ACE) further converts angiotensin I into angiotensin II, a peptide with potent vasoconstrictive properties. Angiotensin II stimulates aldosterone secretion and vasoconstriction. Synthesized in the adrenal cortex, aldosterone is the most potent naturally occurring mineralocorticoid. Through mineralocorticoid receptor binding in the colon and nephron of the kidney, it promotes reabsorption of sodium in the proximal convoluted tubule and potassium and hydrogen ion excretion. The combined effects of aldosterone and downstream effects of angiotensin II increase circulating blood volume and blood pressure.

Sonic Reference Laboratory’s Renin Activity assay quantitatively measures the activity of renin in plasma. Because many factors, including age, posture, sodium/potassium balance, time of day, menstrual cycle, medications, and conditions of blood collection, among others, influence the renin-aldosterone system, evaluation of renin activity should be performed under controlled conditions. Samples should be collected mid-morning after the patient has been active for at least two hours, and the patient has been seated for 5-15 minutes.
Ordering Recommendation
Aid in the diagnosis and treatment of primary and secondary aldosteronism.
Compliance Statement
This test was developed and its performance characteristics determined by Sonic Reference Laboratory (SRL).
It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes and should not be regarded as investigational or for research. SRL is qualified to perform
high complexity testing under the Clinical Laboratory Improvement Amendments (CLIA).
Notes: Replaces E006