Test Catalog

Test

Code
U854
Name
HSV 1/2 BY PCR (HERPES SIMPLEX VIRUS 1/2)
Description

Alternative Names: U854
Includes: HSV 1 Interp, HSV 2 Interp, Specimen Type, Source Site
Sample Type: Swab (skin and mucous membrane lesions), Blood* (Plasma, Serum, Whole Blood (EDTA)) or Body Fluid* (Amniotic Fluid, Bronchoalveolar Lavage (BAL), Cerebrospinal Fluid (CSF), Ocular Fluid)
Preferred Container: See Handling Instructions
Handling Instructions: See Handling Instructions

Body Fluids* (Amniotic Fluid, Bronchoalveolar Lavage (BAL), Cerebrospinal Fluid (CSF), Ocular Fluid)
Sample Volume: 2mL
Transport: Refrigerated or Frozen (Preferably Frozen)
Stability: Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, place immediately into a sterile leak proof container. Do not transfer body fluids to transport or preservative media. Refrigerate or freeze immediately after collection. Freezing is strongly recommended.

Blood Samples* (Whole Blood: Collect in EDTA (Lavender Top) Tube, Plasma: Collect in EDTA (Lavender Top) Tube, Serum: Collect in Serum Separator Tube)
Sample Volume: 2 mL
Transport: Refrigerated or Frozen
Stability: Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Collect specimen per tube manufacturer’s instructions. For plasma or serum samples, centrifuge tube and separate plasma or serum from cells ASAP. Refrigerate or freeze immediately after collection.

Swabs/Collection Devices*
Viral/Universal Transport Media (VTM/UTM) or equivalent
Sample Volume: One swab in 2-3 mL media
Transport: Refrigerated or Frozen
Stability: Ambient: 3 Days; Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, it should be placed immediately into the transport vial where it comes in to contact with transport media.

Aptima Multitest/Vaginal Collection Container/ Swab (Orange)
Sample Volume: One swab in 2-3 mL media
Transport: Ambient or Refrigerated
Stability: Ambient: 2 Weeks; Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, it should be placed immediately into the transport vial where it comes in to contact with transport media.

ESwab Collection and Transport System (White)
Sample Volume: One swab in 1 mL media
Transport: Ambient or Refrigerated
Stability: Ambient: 2 Weeks; Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, it should be placed immediately into the transport vial where it comes in to contact with transport media.

Roche cobas PCR Media*
Sample Volume: One swab in 4 mL media
Transport: Ambient or Refrigerated
Stability: Ambient: 2 Weeks; Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, it should be placed immediately into the transport vial where it comes in to contact with transport media.

ThinPrep or SurePath
Sample Volume: 2 mL media
Transport: Ambient or Refrigerated
Stability: Ambient: 2 Weeks; Refrigerated: 2 Weeks; Frozen: 1 Month
Collection Instructions: Once specimen is collected, it should be placed immediately into the transport vial where it comes in to contact with transport media.
Transport Temperature: See Handling Instructions
Specimen Stability: See Handling Instructions
Testing Frequency: Monday, Tuesday, Wednesday, Thursday, Friday
Expected Turn Around Time: 5 – 7 Days
Testing Facility: SRL
CPT Codes: 87529 X 2
Additional Information: Herpes simplex viruses (HSV-1 and-2) are associated with a wide variety of clinical conditions including genital infections, recurrent mucocutaneous infections, herpetic whitlow, keratitis, aseptic meningitis, encephalitis, neonatal herpes and visceral infections. HSV-1 is more prevalent in oral infections and HSV-2 is more prevalent in genital infections, although both viruses may infect the same areas of the body. Following initial HSV infection, the virus is maintained in a latent state in the infected host, and recurrent infections may occur. The anatomical site, severity, duration and frequency of recurrent HSV infection are highly dependent on the type of HSV. For appropriate clinical management, prevention of transmission and complications, and patient counseling, laboratory HSV typing may be useful. Diagnosis may be confirmed by PCR detection of HSV DNA in patient samples. PCR-based tests have greater sensitivity and specificity than viral culture.

Compliance Statement: This test was developed and its performance characteristics determined by Sonic Reference Laboratory (SRL). It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes and should not be regarded as investigational or for research. SRL is qualified to perform high complexity testing under the Clinical Laboratory Improvement Amendments (CLIA).