Test Catalog

Test

Code
F269
Name
ANTIPHOSPHOLIPID SYNDROME REFLEXIVE PANEL
Description

Alternative Names: F269, APS, CLA, LUPUS ANTICOAGULANT, LUPUS INHIBITOR, 7079
Includes: CARDIOLIPIN AB-IGG, CARDIOLIPIN AB-IGM, B2 GLYCOPROTEIN-1 IGG, B2 GLYCOPROTEIN-1 IGM, PROTHROMBIN TIME, INR, PARTIAL THROMBOPLASTIN TIME. DRVV SCREEN
***Others are REFLEXED, as needed:***
1:1 PTT MIXING, THROMBIN TIME, REPTILASE , PTT HEPARIN NEUT, DRVVT SCREEN RATIO, 1:1 DRVVT SCREEN MIX, DRVV CONFIRM , DRVVT CONFIRM RATIO ,DRVVT NORMALIZE, ATIO, HEX PHOSP NEUT DELTA
Sample Type: Citrated Platelet Poor Plasma (PPP), see below for instructions
Preferred Container: Sodium Citrate (Blue Top, 3.2%) Tube
Sample Volume: 6. 1 mL aliquots of Platelet Poor Plasma
Handling Instructions:
1. Draw 6 blue top tubes (3.2% sodium citrate), properly filled, as the appropriate ratio of blood to anticoagulant is critical to testing. Fill a light blue top tube as far as vacuum will allow and mix by gentle inversion five to six times. The exact ratio of 1 part sodium citrate to 9 parts blood must be maintained.
2. Patients who have hematocrit values above 55% should have the anticoagulant adjusted to maintain the 1 to 9 ratio. For further instructions on the collection of platelet-poor plasma for patients with hematocrit value of >55%, please see specimen collection manual on AEL website.
3. Spin sample to obtain platelet poor plasma (PPP) 2000-2500 x g for 15 minutes(or per centrifuge manufacturer’s collection instructions)
4. Aliquot approximately 3/4 of plasma from the top of new tubes, being careful not to touch the buffy coat.
5. Spin again according to specifications above and aliquot (again avoiding the buffy coat) into 6 plastic vials, each containing at least 1 ml of platelet poor plasma (PPP).
6. Immediately freeze aliquots. Do not thaw and refreeze.
7. The specimens must be separated and frozen within 4 hours of collection.
8. The double spin procedure described above ensures that the plasma is platelet free (<10x10x9/L). Test results are a direct reflection of sample integrity
Transport Temperature: Critical Frozen
Specimen Stability: Frozen: 2 months
Testing Frequency: Twice Weekly
Expected Turn Around Time: 5-9 days
CPT Codes: 85610,, 85613, 85730, 86147 X2, 86146 X2; if reflexed, additional CPT codes may apply: 85670, 85635, 85730- additional, 85525, 85613-additional X2, 85598, Pathologist Consult 85390-26
Note: When multiple tests are ordered, submit separate tubes for this test.
-If PT, PTT and DRVV screening tests along with IGG/IGM antibodies are normal, interpretive comment will be provided.
-If any test results are abnormal, the results will be reviewed by a pathologist (coagulation consultant) with an interpretation provided.
CPT code 85390-26 will apply.
Submission of anticoagulant use will assist in interpretation. Anticoagulants Code :Description ACOVA: Acova (argatroban) ANGIOMAX: Angiomax (bivalirudin) COUMADIN: Coumadin (warfarin) ELIQUIS: Eliquis (apixaban) HEP-SC: Heparin (SC) HEP-UFH: Heparin (UFH) LMWH: LMWH (e.g. Lovenox) NONE: None (no known anticoagulant) OTHER: Other – PRADAXA: Pradaxa (dabigatran) XARELTO: Xarelto (rivaroxaban)