Also known as: t(15, 17) by RT-PCR QUANTITATIVE, PMRAP, W087
Test Contains (LOINC): PML-RARA Translocation t(15;17) (21551-7), PML-RARA Translocation t(15;17) Quant (21785-1)
Sample Type: Whole Blood or Bone Marrow
Preferred Container: EDTA (Lavender Top) Tube or Bone Marrow (EDTA)
Sample Volume: Whole Blood: 5.0 mL OR Bone Marrow:3.0 mL
Minimal Sample Volume: 1.0 mL
Transport 5 mL whole blood (Min: 1 mL). OR 3 mL bone marrow (Min: 1 mL). Specimens must be received within 48 hours of collection due to lability of RNA. CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Client Transport Temperature: Refrigerated
Specimen Stability: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Unsuitable Specimen: Serum, plasma, ambient or frozen bone marrow or whole blood, CSF, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens. Ambient whole blood and ambient bone marrow specimens past 7 days will be cancelled. Refrigerated whole blood or bone marrow past 7 days will be canceled.
Frequency: RNA Isolation: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday Assay: Varies
Expected Turn Around: 3 – 10 Days
Responsible Dept: Reference Lab
Referral Lab Code: 2002871
Methodology: Reverse Transcription Polymerase Chain Reaction
Notes: This assay qualifies as COG approved for the AAML0631 study.