- PML-RARA TRANSLOCATION T(15 17) BY RT-PCR QUANTITATIVE
Also known as: t(15, 17) by RT-PCR QUANTITATIVE, PMRAP, W087
Orderable Code: W087
Test Contains (LOINC): PML-RARA Translocation t(15;17) (21551-7), PML-RARA Translocation t(15;17) Quant (21785-1)
Sample Type: Whole Blood or Bone Marrow
Preferred Container: EDTA (Lavender Top) Tube or Bone Marrow (EDTA)
Sample Volume: Whole Blood: 5.0 mL OR Bone Marrow:3.0 mL
Minimal Sample Volume: 1.0 mL
Handling Instructions: Transport 5 mL whole blood (Min: 1 mL). OR 3 mL bone marrow (Min: 1 mL). Specimens must be received within 48 hours of collection due to lability of RNA.
CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Client Transport Temperature: Refrigerated
Specimen Stability: Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Unsuitable Specimen: Serum or plasma. Frozen or clotted specimens. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed specimens.
Frequency: RNA ISOLATION: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday ASSAY: Sunday, Tuesday, Thursday
Expected Turn Around: 3 – 8 Days
Responsible Dept: Reference Lab
Referral Lab: ARUP
Referral Lab Code: 2002871
Methodology: Reverse Transcription Polymerase Chain Reaction
Notes: This assay qualifies as COG approved for the AAML0631 study.